CHEMICAL PROFILING OF ANTIMICROBIAL COMPOUNDS FROM STREPTOMYCES SP.

Renu Solanki, Aditi Kundub, Payal Das and Monisha Khanna*

ABSTRACT

Natural products from micro-organisms are an important source of antibiotics against drug resistant pathogenic microbes. Actinomycete genera especially Streptomyces are well known for production of antimicrobial compounds. In the present study, Streptomyces sp. isolated from HCH dump site, Lucknow was subjected to antimicrobial analyses. Structure elucidation of purified bioactive compounds was done by mass spectrometry (MS) and nuclear magnetic resonance (NMR) after their identification by bioautography. It was revealed that compound structures showed partial resemblance to known antibiotic groups indicating tentative presence of novel chemical moieties. One of the compounds from isolate L3.46 extracted in ethyl acetate had molecular weight of 681g/mol similar to mycinamicin III, a macrolide antibiotic. Mass fragmentation pattern of L3.46 compound showed partial resemblance to mycinamicin III. A second bioactive compound isolated from L3.46 extracted in methanol was found to have molecular weight of 771 g/mol. Its core structure showed resemblance to xantholipin, belonging to xanthone group of antibiotics. On the basis of structural studies, compounds were identified as Mycinamicin III and Xantholipin derivatives respectively.

Keywords: Actinomycetes, Antimicrobial compounds, Structure elucidation, Mass spectrometry (MS), Nuclear magnetic resonance (NMR)