EVALUATION OF PHYTOCHEMICALS, ANTIOXIDANT POTENTIAL AND ANTIBACTERIAL ACTIVITY OF SIDDHA VEPPAMPATTAI THAILAM PRESCRIBED TO CURE WOUND, KANYAKUMARI DISTRICT - INDIA

R. Mary Suja* and B. Christudhas Williams

ABSTRACT

The main objective of the present investigation is to evaluate the phytochemical constituents, antioxidant potential and antibacterial activity of Siddha wound medicine prescribed by the Traditional Siddha Practitioner of Kanyakumari District, India. Veppampattai Thailam is an internal form of wound medicine. Phytochemical analysis of the Thailam, aqueous, ethanol and chloroform extract revealed the presence of alkaloid, saponin, reducing sugar and tannin constituents. The unexplored area of hydroxyl radical scavenging assay of Veppampattai Thailam and extracts varied from the minimum inhibition 48.57% (25μl) of aqueous extract to the maximum inhibition 84.21 % (100μl) of Ascorbic acid. On the otherhand, reducing power activity of the Thailam and extracts varied from the minimum inhibition 35.53 % (25μl) of aqueous extract to the maximum inhibition 86.79 % (100μl) of Vitamin C. The antioxidant assay of the Veppampattai Thailam and extracts indicated promising antioxidant activities in concentration dependent manner. The antibacterial activity of Siddha medicine prescribed for wound (Thailam) showed both positive and negative activity. The prescribed Veppampathi Thailam showed the maximum zone of inhibition (26 ± 0.001 mm) against Pseudomonas aeruginosa and minimum zone of inhibition (1 ± 0.213 mm) against Klebsiella pneumonia. On the other hand, ethanolic extract of the Thailam fail to inhibit the growth of Klebsiella pneumonia. The trial Siddha medicine highlights the effect of Veppampattai Thailam as a great potential source to rid wounds.

Keywords: Veppampattai Thailam, phytochemical, antioxidant, Kalanchi.