EVALUATION OF IN VITRO LIPID PEROXIDATION INHIBITION ACTIVITY OF HEPATOPROTECTIVE POLYHERBAL FORMULATION

Jayachandra Kuncha*, Thirugnanasambantham P., Kumaran S., Narayanan N. and Sharmila Devi V.

ABSTRACT

The free radicals are the agents which causes cell membrane damage which leads to formation of lipid hydroxyl and peroxy radicals. This is the primary process in cellular damage. The lipid peroxidation process can preventing through supplying scavengers of free radicals. This is the ultimate reason for supplying antioxidants. Botanicals are the wide source of antioxidants available in nature. The main objective of this study is to evaluate anti-lipid peroxidation activity of the hepatoprotective polyherbal formulation (LVR05) by in vitro method. The results shows effective anti-lipid peroxidation activity in dose dependent manner. The maximum lipid peroxidation inhibition was 80.89% at the concentration of 500 μg/ml, at the same concentration standard ascorbic acid shows 91.75% of inhibition. The IC50 values of sample and standard was found 112 μg/ml and 65.74 μg/ml. Based on the results it has been concluded that the polyherbal formulation is effective in prevention of lipid peroxidation. Further work is in progress towards identification and quantification of phytochemicals present in the formulation.

Keywords: In vitro Lipid peroxidation inhibition, Polyherbal formulation, Free radicals, Phytochemicals.