ANTICANCER ACTIVITY OF THYMOQUINONE AND ALLICIN ON CANCER PROSTATE (PC3) AND COLON CANCER (CACO2) CELL LINES, IN VITRO STUDY

Elsayed Mohamed Mahdy, Sara Mohamed Abdu, Mohamed Ali Abd El Baseer and *Waleed Abdelwanees Mohamed

ABSTRACT

Thymoquinone and Allicine, natural products extracted from black seeds and garlic, respectively, are known as having anticancer activity. The activity of these two natural products was investigated on cancer prostate (PC3) cells and colon cancer (CaCO2) cells. The IC50 of these two compounds on PC3 cells was found to be 623.63 and 10.87 μg/ml, respectively and on CaCO2 cells was found to be 389.60 and 20.46 μg/ml, respectively. When using these concentrations in cell cycle analysis, it was found that the percent of G2-M phase and apoptosis was increased, while the percent of S phase was reduced for cells treated with these two compounds compared to control, untreated cells. Real time RT-PCR revealed that the activity of P53, caspase 3 and survivin was up-regulated, while the activity of Bcl2 was down-regulated compared to control cells. These results indicate that thymoquinone and Allicin have anticancer activity against cancer prostate (PC3) cells and colon cancer (CaCO2) cells.

Keywords: Thymoquinone, Allicin, PC3, Caco2.