EFFECT OF WHOLE CELL AND OUTER MEMBRANE PROTEIN VACCINES ALONG WITH IMMUNOADJUVANT ANDROGRAPHIS PANICULATA AGAINST STAPHYLOCOCCOSIS DISEASE IN FINGERLINGS OF LABEO ROHITA

Deepalakshmi R.* and Brindha Devi G. B.

ABSTRACT

Whole cell and Outer membrane protein vaccines, when administered with immunoadjuvant increases the efficacy of the vaccine uptake and boosts the innate immune system of fish. The present study is to develop the whole cell and outer membrane protein vaccine, administered along with immunoadjuvant Andrographis paniculata in Labeo rohita fingerlings, post challenged with virulent Staphylococcus aureus. 25 fingerlings were pre-treated with hyper- osmotic solution of 2% NaCl and vaccinated twice with booster dose on day 1 and second dose on day 30. After 30 and 60 dpv (days post vaccination) fingerlings were bath challenged for up-to 1 hour in the lethal dose of 1x108 cells/ml. SDS-PAGE results revealed, WC vaccine with band length of 27.95 KDa and OMP vaccine with band length of 27.43-35.43 KDa respectively. Total protein concentration of WC vaccine was found to be 34μg/ml and 22μg/ml in OMP vaccine respectively. After 30 and 60 dpv there was significant increase in RPS rate of experimental groups, showing a RPS 84% in OMP and 92% in OMP+A when compared to a RPS of 76% in WC and 80% in WC+A respectively. The results inferred that, both OMP and WC vaccine along with an immunoadjuvant could be effective vaccine candidates against staphylococcosis disease in Labeo rohita fingerlings.

Keywords: Labeo rohita, Whole cell, Outer membrane protein, Staphylococcus aureus, immunoadjuvant, Andrographis paniculata, mortality, relative percent survival.