“INVESTIGATION THE ANTILITHIATIC POTENTIAL OF HORSE GRAM (MACROTYLOMA UNIFLORUM) SEEDS EXTRACTS ON EXPERIMENTAL KIDNEY STONES FROM HIGH ALTITUDE OF UTTARAKHAND (INDIA)”

Netrapal Sharma*, Satpal Singh Bisht, Sanjay Gupta and Ajay Kumar

ABSTRACT

The stones in kidney are also known as a calculus and crystal aggregation formed by dietary minerals in the urine. Lithiasis is a multifaceted progression that occurs from series of several physicochemical event including supersaturation, nucleation, growth, aggregation and retention within the kidneys. Many plant species of horse gram (Macrotyloma uniflorum) have been reported to posses anti-lithiatic property. In the present study aqueous, ethanolic and methanolic extracts and total seed protein of M. uniflorum and standard for dissolving kidney stones (collected from hospitals) and calcium oxalate crystals (in-vitro prepared). To confirm their anti-lithiatic potential to dissolve calcium oxalate crystals and kidney stones by an in-vitro model for M. uniflorum seeds and cystone as a standard compound collected from market. Total protein was showing significant effective in dissolving of calcium oxalate (80%) and kidney stones (65%). Aqueous fractions showed highest dissolution of stones and calcium oxalate crystals as compare to others. Aqueous fraction was more effective in dissolving calcium oxalate (85%) and kidney stones (84%). Standard-formulation Cystone was found to be more effective (87 %) when compared to others. The outcome of the study on the stone-forming minerals will provide a good indication of the risk management of stone formation. This study has given primary evidence for Macrotyloma uniflorum as plant which possess good anti-lithiatic property.

Keywords: Macrotyloma uniflorum, Kidney Stones, Anti-lithiatic, Calcium Oxalate, Total Protein.