EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF CITRUS LIMON LINN. (LEAVES)

Vijeta Gupta*, Dr. Vijender Singh and Dr. Mojahidul Islam

ABSTRACT

Citrus limon linn belonging to Rutaceae family is highly reputed plant and has been widely employed in herbal medicine and aromatherapy but no work has been carried out on the antioxidant activity of the leaves extracts. The antioxidant property of methanolic extract of Citrus limon Linn. was evaluated by estimation of Total Flavonoid content (TFC), Free radical scavenging activity (DPPH- radical scavenging activity) and Total Phenolic content (TPC). The Total flavonoid content was measured by Aluminum chloride colorimetric method at 420 nm and was expressed as mg quercetein equivalent (QE)/gm dry plant material. The DPPH radical scavenging activity of C.limon Linn was estimated by Inhibitory concentration (IC50) at 517 nm and the Total Phenolic content was analyzed by Folin - ciocalteu colorimetric method using gallic acid as standard (GAE), absorbance was reported at 765 nm. Results were reported as Total Flavonoid content was 3.73 mg/gm QE (quercetin equivalent), DPPH radical scavenging activity (IC50 value) was 310 μg/ml and Total Phenolic content was 1.96 mg/gm GAE (gallic acid equivalent). Thus from ongoing study it is concluded that leaves of Citrus limon Linn. possess significant Antioxidant activity and can be used for further research study.

Keywords: Anti oxidant activity, Citrus limon Linn.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database