DEVELOPMENT OF UV- SPECTROPHOTOMETRY METHODS FOR ESTIMATION OF AGOMELATINE IN BULK AND IN TABLET FORMULATION

Vinod A. Chaure*, Ashwini V. Gujar, Dr. Dheeraj H. Nagore and Dr. Atul A. Shirkhedkar

ABSTRACT

Four Simple, precise and economical UV-Spectrophotometric method has been established for the quantification of Agomelatine in bulk and Tablet Formulation. Method A is Zero order UV-Spectrophotometry using absorbance, Method B is zero order UV-Spectrophotometry using AUC, Method C is First order derivative UVSpectrophotometry using amplitude and Method D is First order derivative UV-Spectrophotometry using AUC, The proposed methods were successfully applied for the determination of Agomelatine in bulk and tablet formulation. In methanol Agomelatine showed maximum absorbance at 276 nm. In Method A, absorbance was recorded at 276 nm while in Method B, AUC was selected in the wavelength range of 271.4 - 279.8 nm, In Method C, the amplitude was recorded at 279 nm while in Method D, AUC of the derivatized spectrum was studied at 276.2 – 282.6 nm. In all the Methods, Agomelatine followed linearity in the concentration range of 5 - 30 μg/mL with (R2> 0.999). All these developed methods were applied for estimation of Agomelatine in tablet formulation. All these methods were validated for linearity and range, accuracy, precision, ruggedness and sensitivity.

Keywords: Agomelatine, UV Spectrophotometry, Zero Order, AUC, First Order Derivative.