GC/MS ANALYSIS PRODUCTS AND THEIR RELATION WITH THE ANTIMICROBIAL ACTIVITY OF LEAVES AND BARK OF CAPPARIS SPINOSA SUBSP ORIENTALIS (DUH.) JAFRI

Salwa Ibraheim El-Tawaty*, Mohamed-Elfatih Ahmed Omare, Aisha Zoheir Almagboul and Sanaa Osman Yagoub

ABSTRACT

This study aimed to analyze the phytochemical constituents and investigate the antimicrobial activity of Capparis spinose crude bark and leaves extracts against different microorganisms. Methods: Gas Chromatography/ Mass Spectra (GC/MS), Disc diffusion method and dilution agar plate assays were used for analysis and to evaluate the antimicrobial activities and minimum inhibitory concentrations. Results: Extracts of Capparis spinosa leaves and bark from different solvents showed variable in – vitro activity against tested standard strains and clinical isolates with highly significant differences; P˂ 0.01. The highest activities were shown from chloroform bark extract and methanol leaves extract with equal minimum inhibitory concentration of 100mg/ml against standard Staphylococcus aureus ATCC 25923 and clinical methicillin resistant Staphylococcus aureus. GC/MS analysis revealed 33 and 25 compounds from extracts of the plant leaves and bark, respectively and the study suggested that the antimicrobial activity shown is referred to these phytochemical constituents such as flavonoids, tannins, steroids and triterpines. Conclusion: Capparis spinose subsp orientalis (Duh. Jafri leaves and bark extracts composed of flavonoids, tannins, steroids and triterpines which have the ability to reduce surface colonization and to control the penicillin binding proteins of Staphylococcus aureus, and this make the plant have a pronouncing anti Staphylococcus aureus especially with the limited treatment choices presented for treatment of infections caused by this bacterium.

Keywords: Capparis spinosa, GC/MS analysis, phytochemical constituents, Staphylococcus aureus.