EFFECT OF ORGANIC NITROGEN SOURCE FOR PRODUCTION OF FIBRINOLYTIC ENZYME AS A “CLOT BUSTING DRUG BY ASPERGILLUS JAPONICUM

Saroj Yadav* and Siddalingeshwara K. G.

ABSTRACT

Cardiovascular disease (CVDs) is caused by disorders of the heart and blood vessels, and includes coronary heart disease (heart attacks), cerebrovascular disease (stroke), raised blood pressure (hypertension), peripheral artery disease, rheumatic heart disease, congenital heart disease and heart failure. Fibrinolytic enzymes are agents that dissolve fibrin clots extracted from microbes were considered to be a potential thrombolytic agent for the treatment of clot-dissolving in the cases of Myocardial infarction. The fungal strains i.e Aspergillus japonicum KGSY 05 were isolated from different soils from different regions of Karnataka and screened for fibrinolytic activity by plate assay. The potential strain Aspergillus japonicum KGSY 05 were used for the enhance the biosynthesis of fibrinolytic enzyme by supplementation of nitrogen (organic) sources in range of 0.25% to 1.25%. The different organic nitrogen sources such as yeast extract, beef extract and peptone were employed. The peptone showed better yield of 123 IU fibrinolytic enzyme production and yeast extract (1.0%) 68 IU, beef extract (1.0%) 103 IU.

Keywords: Clot buster drug, fibrin carbon source, Aspergillus tamari.