ANTITUMOR ACTIVITY AND ANTIOXIDANT STATUS OF RETICULATACIN AGAINST TESTOSTERONE INDUCED PROSTATE CANCER IN ALBINO WISTAR RAT

Saritha Surapaneni*, T. Prakash and Dinesh Ghatamaneni

ABSTRACT

The hydroalcoholic extract of Annona reticulata. L Bark powder was extracted by successive extraction with pet ether, chloroform and ethanol solvents and hydro alcoholic extract was collected and phytochemical evaluation are carried and isolation of reticulatacin and characteristics of active constituents by FTIR & UV Spectroscopy, HPLC, Mass Spectroscopy, 1H-NMR and 13C-NMR. Evaluation of antitumor activity against testosterone-induced prostate cancer (PC) in albino wistar rats and reticulatacin was administered for Healthy adults male Wistar albino rats were divided into five groups (n = 8). Group I was served as vehicle control (Arachis oil 1ml/kg, SC), group II was served as testosterone Depot (TD) injection (3 mg/kg, s.c in Arachis oil), group III was served as standard Finasteride (5 mg/kg, p.o, and TD 3 mg/kg, s.c in Arachis oil), group IV was served as treated with reticulatacin (50 mg/kg p.o and TD 3 mg/kg, s.c in Arachis oil), group V reticulatacin (100 mg/kg p.o and TD 3 mg/kg, s.c in Arachis oil) for 28 days to assess the preventive effect of Reticulatacin. After the treatment animal was sacrificed and the prostate was removed and homogenized. Estimation of lipid peroxidation (LOP), reduced glutathione (GSH), glutathione peroxidase activity (GPx), glutathione Reductase (GR), Catalase (CA), estimation of protein levels and the histopathological changes were observed. Reticulatacin caused a marked decrease in prostate weight of prostate cancer-induced rats whereas glandular hyperplasia occurred in the negative control. Decreased the levels of lipid peroxidation and significantly (P < 0.05) increased the levels of GSH, GPx, and CAT recorded a marked increase in the levels of antioxidant enzymes compared to the negative control. It also exhibited antioxidant properties and showed to be a good prophylaxis.

Keywords: Reticulatacin, prostate cancer, benign hyperplasia Testosterone Depot, biochemical parameter, histopathology, antioxidant, antitumor activity.