EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

DETECTION OF BIOFILM IN COAGULASE NEGATIVE STAPHYLOCOCCUS: COMPARISION OF PHENOTYPIC METHODS

Dr. Smruti Mohanty and Dr. Laxmi Kant Mohanty*

ABSTRACT

Coagulase negative Staphylococci (CoNS) are a major component of the normal flora of the cutaneous ecosystem and act as commensal or saprophytic organisms. The ability to form biofilm plays an important role in contributing virulence of Coagulase Negative Staphylococcus (CoNS). It is considered as normal commensal and shown to cause infections in neonates and immunocompromised patients, especially with indwelling medical devices. The ability to form biofilm plays an important role in contributing virulence of CoNS. Also CoNS is found to be associated with nosocomial infections. The present study has been planned to detect biofilm production and also to evaluate three phenotypic methods. Aim: To detect biofilm production in CoNS from the clinical samples phenotypically by using: Tissue culture plate method (TCP), Tube adherence method, Congo red agar method (CRA). Materials and Methods: 100 CoNS isolates were subjected to species identification by standard biochemical tests. Biofilm detection of all isolates was done by TCP, CRA and tube method. Results: Of the total 1058 culture positives isolates 100(9.45%) were identified as CoNS. Of the total 100 isolates 54% were identified as S. epidermidis, 14 (14%) were identified as S. haemolyticus, 10 (10%) were identified as S. capitis, 8 (8%) as S. cohinii, and 14 (14%) as S. Saprophyticus. Of the total isolates biofilm production was seen in 62(62%) isolates by Tissue culture plate (TCP). By Congo red agar method biofilm production was 63 (63%). In tube method biofilm production was in 66 (66%) isolates Conclusions: Amongst phenotypic methods CRA was more sensitive and can be easily performed in the routine laboratories laboratory and can be used as a screening test for detection of biofilm.

Keywords: Coagulase negative Staphylococci (CoNS) detection of biofilm.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database