POLYMORPHISMS IN APE1 AND HOGG1 DNA REPAIR GENES AND RISK OF CERVICAL CANCER IN MAHARASHTRIAN WOMEN: A HOSPITAL BASED CASE-CONTROL STUDY

Shreepad A. Joshi, Kailas D. Datkhile*, Madhavi N. Patil, Pratik P. Durgawale, Kalpita S. Korabu and Satish V. Kakade

ABSTRACT

Background: Cervical cancer is a major concern of health risk in urban and rural parts of India. Maharashtrian population is not subjected to investigate cervical cancer susceptibility in association with genetic determinants as risk factor to cause cervical cancer. This study was aimed to find out frequency of polymorphisms in DNA repair genes including Apurinic/apyrimidinic endonuclease 1 (APE1) and 8-oxoguanine DNA glycosylase 1 (OGG1) gene in patients of cervical cancer from Maharashtra, and to evaluate their association with risk of cervical cancer. Methods: We used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to examine gene polymorphisms in 350 patients with cancer of cervix and 400 age and sex matched normal controls. Results: The results obtained indicated that there was no significant difference in the genotype distribution between cervical cancer patients and controls for APE1 Asp148Glu allele of codon 148 in exon 5. The result showed that genotype frequencies of hOGG1 Ser326Cys of codon 326 in exon 7 (OR=5.40; 95% CI= (3.78-7.73); p= <0.0001) were increased significantly. Conclusion: This study indicates that polymorphisms and haplotypes in hOGG1 gene appear to influence genetic susceptibility of individual to cervical cancer in Maharashtrian patients.

Keywords: Cervical cancer, Genetic polymorphism, APE1, hOGG1, PCR-RFLP.