( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



*Ronald Bartzatt and Purnima Gajmer


A method to assay ephedrine hemihydrate (ephedrine alkaloid), which has various biological activities, is presented using isocratic high performance liquid chromatography (HPLC). Elution of the analyte is detected with ultraviolet light detector, set at 255 nm. The stock solution of this ephedrine alkaloid was prepared in solvent conditions that consisted of 10% ethanol (v/v) and 90% water (v/v), at a concentration of 7.1568 x 10-2 molar. The test samples of ephedrine hemihydrate that were injected into HPLC instrument were mainly in a solvent that consisted of 90% (v/v) water and 10% ethanol (v/v). The solvent utilized for column of the HPLC instrument was 5% ethanol, 1% glacial acetic acid (v/v), and 94% water (v/v). The limit of detection (LOD) was found to be 2.9833 x 10-5 molar and the limit of quantitation (LOQ) was found to be 8.9500 x 10-5 molar. A standard curve presented showed a coefficient of determination of R2 = 0.9857, which indicates that the model describes 98.57% variance in the dependent variable (peak area) that is predictable from the independent variable (molar concentration). The Pearson r correlation coefficient of this standard curve is 0.9928, indicating very high positive correlation. Ephedrine hemihydrate is assayed from matrixes that are utilized in the administration of this drug, including water and cellulose for tablet formulation. The determination of the drug ephedrine alkaloid is consistent and accurate utilizing isocratic conditions with HPLC.

Keywords: Ephedrine alkaloid, HPLC, isocratic, ephedrine hemihydrate.

[Full Text Article]



  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 September 2023 Issue has been Published, Kindly check it on


    SEPTEMBER issue has been successfully launched on 1 SEPTEMBER 2023.

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    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from  5.804 to 6.222, due to high quality Publication at International Level

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database