STUDY OF THE URINE AND BLOOD BIOCHEMICAL PARAMETERS OF CONTROLLED DIABETIC NON CALCULOGENIC AND CONTROLLED DIABETIC CALCULOGENIC RATS

Dr. Dhanalekshmy T G*

ABSTRACT

Experimental studies are important as prerequisite to clinical trials. The objective of the present study is to understand the changes produced in the urine and blood biochemical values in the controlled diabetic non-calculogenic and controlled diabetic calculogenic rats. The experimental work was conducted in male rats of wistar species. Diabetes was induced by injecting 3% aqueous solution of alloxan monohydrate in a dose of 150mg/kg body weight and calculogenesis by giving 0.5% of ethylene glycol orally in drinking water. Diabetes was controlled by giving lente insulin. The 24-hour urine samples intermittently and blood at the end of the study was analyzed to assess the urine parameters calcium, phosphorous, uric acid, oxalate, magnesium and citrate and serum parameters calcium, phosphorus, uric acid and magnesium. The mean values of the promoters urine uric acid and serum calcium (p<0.05, p<0.05) and inhibitors urine citrate (p<0.01) and magnesium (p<0.001) and serum magnesium (p<0.01) were significantly lower in controlled diabetic non-calculogenic rats. Controlled diabetic calculogenic rats had significantly low urine calcium (p<0.05), uric acid (p<0.0001) and serum uric acid (p<0.04) and magnesium (p<0.01). Serum phosphorus (p<0.01) was significantly higher while the serum magnesium (p<0.01) was significantly lower in the controlled diabetic calculogenic rats. Comparative study of the non-calculogenic and calculogenic controlled diabetic rats showed serum uric acid to be significantly higher (p<0.05) and urine citrate significantly lower (p<0.05) in the controlled diabetic non-calculogenic rats. Serum magnesium values of the controlled diabetic non-calculogenic and controlled diabetic calculogenic rats were significantly lower. Controlling diabetes of rats showed lower value of urine and serum promoters but did not elevate the levels of inhibitors which indicate that the tendency for stone formation in the diabetic rats is not completely reversed on controlling the diabetic state.

Keywords: Controlled diabetic non-calculogenic (CD NC), Diabetic non-calculogenic (D NC), Diabetic ethylene glycol induced calculogenic (D EG), Calculogenesis.