FORMULATION AND EVALUATION OF POLAXOMER COATED RETROVIRAL NANOPARTICLES

Chamala Aparna*, S. Chellaram and D. Dhachinamoorthi

ABSTRACT

Nanoparticulate delivery systems were found to be attractive mean in the treatment for various chronic diseases. The nanosize facilities improved bioavailability of poorly soluble drugs targeting to cancer to cancer cell to HIV infected cells. The main objective of this work was to investigate the ability of macromolecular protein. Bovine serum albumin (BSA) for functional carrier property to develop into nanoparticles. Lamivudine (LV) is an anti-retroviral drug and effectively to treat hepatitis B viral infections and also in the treatment of acquired immunodeficiency syndrome (AIDS) caused by Human immunodeficiency virus (HIV). In this study.LV was loaded into BSA solvent desolvation technique and the effect of process variable such as drug carrier ration, volume of cross linking agent, gluteraldehyde (GA) on the particle size, zeta- potential and in vitro release characteristics were studied. The particle size distribution of the formulation was in the range from 469-163nm. The entrapment efficiency and loading capacity of total of nine formulations (F1-F6) were in the range of 28-52% and 14-26%. The zeta potential of the formulation F5- was -27.8 than F6-21.4. the decreased surface charge could be attributed to high GA volumes whereas the in vitro release characteristics of F4-F6 extended for 64 hrs. From the study it was concluded that the method selected was able to effectively load the drug. The in vitro release characteristics found to exhibit better release retardation and prolonging the drug therapy and minimization of multiple doses.

Keywords: Lamivudine, Bovine Serum Albumin, Nanoparticles.