NEW COLORIMETRIC METHOD DEVELOPMENT AND VALIDATION OF BENZOCAINE BY VISIBLE SPECTROSCOPY

P. Salomi*, S. Arshiya Banu, B. Gowthami, R. Spandana, S. Sivaiah, M. Rajesh Kumar

ABSTRACT

Background & objectives: Benzocaine is a local anesthetic used to relieve pain caused by conditions like mouth ulcers or sores. The present study has been undertaken in order to develop new simple, rapid efficient and reproducible method for the analysis of Benzocaine by using NED as a coloring reagent. Methods: With the help of NED N-(1-Naphthyl) ethylenediamine reagent a new colorimetric method was developed and the color intensity of the resulting solution is measured by using Visible spectroscopy. Results: The proposed U.V. spectrophotometric method was validated by evaluation of validation parameters. The proposed colorimetric methods give good reproductive results for the estimation of Benzocaine. This is very simple, rapid and nowhere involves complicate sample preparation. Conclusion: It is simple and rapid, selective, precise. Hence, suitable for application in routine analysis of pharmaceutical preparation.

Keywords: Benzocaine, NED, Colorimetry, Validation.