EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

FORMULATION AND EVALUATION OF 3D INJECTABLE BIODEGRADABLE HYDROGEL FOR THE TREATMENT OF PERIODONTAL DISEASES

Luay Thanoon Younis*, Tommy Julianto Bustami, Tara Bai Taiyeb Ali and Mohamed Ibrahim Abu Hassan 

ABSTRACT

Periodontitis is an inflammatory disease resulting in the destruction and loss of tooth-supporting structures (periodontal ligament and alveolar bone). Objective: This study is designed to formulate, and evaluate a degradable bio-adhesive hydrogel contains titrated extract of Centella Asiatica (TECA 3%, w/w) for healing and regeneration of the periodontal cells in-vitro. Methods: The 3D potential hydrogel formulation is composed of pectin (P) and hydroxypropylmethyl cellulose (HPMC) in various concentrations. The hydrogels were formulated in the range of 1-5%, w/w of pectin and 2 - 4% w/w of HPMC. Characterization of the hydrogels was done using rheometer to study the effect of P and HPMC concentrations on hydrogel rheology behaviour. The texture of hydrogel was characterized using texture analyzer to evaluate its syringeability, firmness, adhesiveness and cohesiveness. Fibroblast proliferation assay was done to assess the effect of the gel on the cells proliferation. Southern blot assay was also done to ascertain the effect of the gel on maintaining fibroblast telomere length during inflammation. Results: The experiments showed that the hydrogels containing higher P and HPMC have potential properties to deliver active compound. Incorporation of TECA in the hydrogels formulations does not affect the physical properties of the hydrogel. One of the best hydrogel formulations with high content of pectin and HPMC were selected. Cell proliferation assay was done to assess the proliferative effects of TECA on the human periodontal ligament fibroblasts (PDLFs). The parameters depicted significant activity of TECA hydrogel in enhancing the proliferation of fibroblast together with preserving their telomere length during inflammation. Conclusion: Due to the regenerative effects of TECA hydrogel, and the suitable gel mechanical properties achieved, the formulated bioadhesive hydrogel may be used as an adjunct for the treatment of periodontal disease and regeneration of the periodontal structure.

Keywords: Hydrogel, periodontal disease, TECA, fibroblast, telomere length.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )