DEVELOPMENT AND VALIDATION OF A SIMPLE SPECTROSCOPIC METHOD FOR ESTIMATION OF CISPLATIN IN BULK AND IV FORMULATION

Indrayani D. Raut*, Rajendra C. Doijad and Shrinivas K. Mohite

ABSTRACT

Cisplatin is an important drug of class antitumour agents and is widely used in treatment of many malignancies including Testicular, ovarian, neck, lung, head and bladder. It has a low molar absorptivity in the UV region and has no fluorescence. So a derivatizing reaction is required for its detection in bulk and pharmaceutical dosage form. A simple, precise, rapid spectrophotometric method has been developed for the determination of Cisplatin. This method developed by complexation of the drug with orthophenylene Diamine and the absorbance of green colour solution shown in visible range at 705 nm. This method was validated according to ICH guidelines. The drug obeyed Beers law and showed good correlation. The method linearity observed in the range from 2 to 10 𝜇g/mL with a correlation coefficient of 0.998. There was no significant difference in the intraday and interday analysis of Cisplatin determined. The results of analysis were validated with respect to recovery, linearity; Limit of detection and limit of quantitation were found to be satisfactory. The proposed method can be applied in routine quality control in the pharmaceutical industries since it is precise, accurate, simple and economic.

Keywords: Cisplatin, Complexation, Validated, Precision, Recovery.