IN SILICO DOCKING AND IN VITRO ENZYME INHIBITION ACTIVITY OF UNRIPE FRUITS OF FICUS RACEMOSA LINN

P. G. Muralikrishna*, Dr. B. Duraiswamy and Nair N. K.

ABSTRACT

Ficus racemosa, a versatile medicinal plant, is investigated for many biological activities. The plant is a unique source of various types of compounds having diverse chemical structures. The ethnobotanical and reported studies says it is indicated in hyperglycemia and diabetes. In this study it evaluates relationship between various reported terpenoids in fruit of plant and the targeted protein relating to diabetes in human, it involved docking of 3D structures of ligand (compounds) to selected targeted proteins. The in silico docking studies confirmed that the terpenoid contents in the fruit is having good interaction to the specific target proteins, among the terpenoids, the 2 compounds, viz. beta sitosterol and Friedelin are having pronounced docking and interaction with receptors 2QMJ and 2V95 the hyperglycemic targets. In invitro enzyme inhibition studies the extracts of unripe fruits of Ficus racemosa was tested to understand the antihyperglycemic potential. Both extracts were found to inhibit enzymes considerably.The methanol extract showed maximum inhibitory activity with alpha amylase enzyme, whereas aqueous extract signaled a maximum inhibitory action with alpha glucosidase enzyme. Docking study and enzyme inhibition data reveals potent implication of unripe fruits of Ficus racemosa in Hyperglycemia and Diabetes.

Keywords: Hyperglycemia, Ficus racemosa, docking, diabetes mellitus, enzyme inhibition, receptors.