EFFECT OF SHRIMP SHELL EXTRACT NANOPARTICLES ON ORAL SQUAMOUS CELL CARCINOMA CELL LINE: IN VITRO STUDY

Dina Sabry Abd-El-Fattah, Wael Sabry Mohammed and *Marwa Mohammed Ellithy

ABSTRACT

To develop a shrimp shell extract based nanoparticles formulation and assess its effect on the viability and carcinogenic potential of human oral squamous cell carcinoma cell line Hep-2 in vitro. Nanoparticles of chitosan were prepared. Characterization was based on particle size, zeta potential, and morphology. Various concentrations of chitosan nanoparticles (0.3- 0.6- 1- 1.5 mg/ml) were used intreating cells for 48 hours then cell viability was assessed to calculate the IC50. VEGF and TNF-α were measured in plates of cells treated with Chitosan nanoparticles in its IC50 concentration. MTT assay results showed that chitosan nanoparticles exhibited concentration dependant cytotoxicity on the Hep-2 cell line. Chitosan nanoparticles are cytotoxic to Hep-2 cell line in-vitro and decreases the angiogenic marker VEGF and TNF-α in a dose dependant way. So, shrimp shell extract nanoparticles could be used as a natural agent in oral cancer therapy.

Keywords: Shrimp shell, Chitosan, nanoparticles; oral cancer; angiogenesis, TNF-?.