ANALGESIC, ANTI-INFLAMMATORY AND CNS DEPRESSANT ACTIVITIES OF THE METHANOL, ETHYL ACETATE AND CHLOROFORM FRACTIONS OF STEREOSPERMUM PERSONATUM (HASSK.) CHATTERJEE FRUITS IN MICE

Md. Akherul Islam*, Luthfun Nesa, Mohammad Amjad Hossain, Muhammad Mohsin Hossain, Hossain Shima and Md. Abu Hanif

ABSTRACT

The study was designed to evaluate the analgesic, CNS depressant and anti-inflammatory activities of methanol, ethyl acetate and chloroform fraction of Stereospermum personatum (S.Personatum) fruits on Swiss Albino mice. Analgesic activity was performed by acetic acid-induced writhing model and formalin induced licking and biting in mice. Anti-inflammatory effects were done by carrageenan induced hind paw edema. The CNS depressant activity was evaluated by observing the hole cross and opens field tests. The methanol, ethyl acetate & chloroform fractions were investigated by 200mg/kg and 400mg/kg dose. Statistical analysis showed that dose of (200 and 400mg/kg) exhibited (significant* p<0.05) moderate analgesic activity (43.16% and 59.35%), (46.76% and 65.46%) and (51.43% and 77.33%) for methanol, ethyl acetate and chloroform fractions respectively against acetic acid induced pain in mice compared to standard (87.05%) and formalin induced exhibited (significant* p<0.05) highly analgesic activity (66.66% and 86.95%), (72.46% and 79.85%) and (84.05% and 94.20%) respectively. Furthermore, Late phase methanol, ethyl acetate and chloroform (200mg/kg and 400mg/kg) higher than standard drug diclofenac sodium (56.52%). Methanol ethyl acetate and chloroform (200mg/kg and 400mg/kg) showed significant anti-inflammatory activity at 1st h to 4th hours. On the other hand, Fractions (200mg/kg and 400mg/kg) showed significant (*p<0.05) depressant activity at 30min to 120min in the hole cross and open field test. This study focused that the effect of methanol, ethyl acetate and chloroform fractions of S. personatum fruits (400mg/kg) dose has shown significant analgesic, CNS depressant action and moderate anti-inflammatory activity.

Keywords: Stereospermum personatum; Analgesic; anti-inflammatory; CNS Depressant; Methanol; Ethyl acetate; Chloroform.