ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT FROM INDONESIAN Curcuma heyneana RHIZOME
D. U. C. Rahayu, Silmi Nurul Adilah and P. Sugita*
Objectives: Temu giring (Curcuma heyneana) is widely used as herbal medicinal plant to treat stomach aches and skin diseases. The objectives of this study are to report the chemical structure and antioxidant activity of the polar fraction of this particular species. Material and Methods: The crude methanol extract was fractionated by vacuum liquid chromatography with gradient elution to produce 10 fractions. Fraction F and H were separated by various chromatography technique giving F121 and H13. Results: Identification of F121 and H13 with liquid chromatography–mass spectrophotometry (LC-MS) showed 3 compounds in F121 and 2 compounds in H13. The compounds were identified as curcumanolide A, (R)-ar-turmerone, (1(10)-E,4β,5α,8β)-4,5-epoxy-1(10),11(13)-germacradien-12,8-olide, Dihydrosuberenol, and demethoxycurcumin. Dihydrosuberenol had been reported in this species, while the other compounds had been found in the same genus. The result of the antioxidant activity toward 1,1-diphenyl-2-picrylhydrazil (DPPH) showed moderate activity of H13 and its crude methanol extract with IC50 59.18 and 82.16 μg/mL, respectively, while the F121 showed a low antioxidant activity with IC50 value > 100 μg/mL. Conclusions: analysis of Indonesian C. heyneana rhizomes gave sesquiterpenes of germacrane, cyclic sesquiterpenoid types, and coumarine compounds and have potency as antioxidant activity.
Keywords: Curcuma heyneana, germacrane, cyclic sesquiterpenoid, coumarine, antioxidant activity, IC50.
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