ISOLATION AND ANTIBIOTICS PATTERN OF SALMONELLA TYPHI FROM FRUIT JUICE

Vishal Kumar Deb, Usha Verma and Ajay Kumar*

ABSTRACT

Salmonellosis is a disease caused by a large group of bacteria of the genus salmonella that can affect human being throughout the world. It is among the most commonly isolated food borne pathogens associated with fresh fruits and vegetables etc. Outbreaks of Salmonellosis have been linked to a wide variety of fresh fruits and vegetables including apple, mango, tomato, melon and parsley. In the present study the isolation of Salmonella was carried by direct plate method from fruit juice, tap water and blood sample on SS Agar. The Salmonella shows small, circular, jet black colonies in fruit juice sample and further screened on differential media such as Bismuth sulphite agar shows silver black colonies and Brilliant green agar shows pinkish white colonies. The isolated strains was G-ve, rod shaped bacteria and shows positive MR, H2S, Catalase, Oxidase, Nitrate reduction test. The isolated strains shows maximum zone of inhibition against ciprofloxacin i.e.35mm, Chloremphenicol i.e.20mm and minimum against Ampicillin i.e. 5mm. The choice of the antibiotics for the treatment of salmonellosis are broad spectrum drugs.

Keywords: Salmonella, Antibiotics, infection.