STANDARDIZATION AND ANTIBACTERIAL ACTIVITY OF SPHAERANTHUS AMARANTHOIDES LINN.

Sathya D, Thiripurasundari B, Ganesan R* and Elankani P

ABSTRACT

The present work is aimed at exploring the physic-chemical parameter, Preliminary phytochemical analysis, TLC Photo documentation, HPTLC fingerprint, Heavy metal analysis of Lead, Cadmium, Mercury and Arsenic, and antibacterial activity of ethanolic extract of whole plant of Sphaeranthus amaranthoides Linn. (Fam: Asteraceae). The physicochemical screening was carried out and loss on drying of the sample ,total ash content, acid-insoluble ash, water& alcohol soluble extractive values of the sample were estimated. The preliminary phytochemical screening was performed and the presence of alkaloids, aminoacids, flavonoids, glycosides, phenol, steroid, tannin, triterpenoid and saponin were observed. HPTLC fingerprinting showed 9 peaks at 254 nm, the TLC photo documentation showed 7 visible spots under 254nm, 9 spots under 366nm and 9 spots after derivatization. Heavy metals such as Cadmium, Mercury and Arsenic were not detected and lead content was within the permissible limit. The antibacterial activity of methanolic extract of whole plant of Sphaeranthus amaranthoides Linn.was carried out against gram positive and gram negative bacteria. The antibacterial activity studied is expressed in terms of zone of inhibition measured in mm. A significant growth inhibition was shown by many of the organisms tested indicating the profound potency of the plant drug. Among the tested organism Bacillus subtilus was found to be the most sensitive organism followed by Escherichia coli, Pseudomonas aeruginosa and Klebsiella Pneumonia., with maximum zone of diameter ranging from 32mm to 9 mm.

Keywords: Sphaeranthus amaranthoides Linn., Standardization, HPTLC, Heavy metals antibacterial activity.