BIOCHEMICAL STUDIES OF FRESH WATER MALE CRAB BARYTELPHUSA GUERINI INOCULATED WITH E.COLI AND STAPHYLOCOCCUS AUREUS

Ch. Tulasi Mastanamma*

ABSTRACT

Carbohydrates, lipids and proteins are found in different forms in various cells as as degenerative material in many crustaceans. In this study total free sugar content, total protein and lipid content of Barytelphusa guerini were estimated using standard protocols. The protein content increase was moderate in the hemolymph of challenged crabs during 2hrs and 6hrs but reached maximum during 12hrs and started decreasing from 12hrs to 24 hrs and 48 hrs gradually in case of male crabs challenged with Gram positive and Gram Negative bacteria. Based on the results it was observed that the carbohydrate content decreased gradually from 2 hrs to 48 hrs and the percentage of decrease is highest, 26% in male crab challenged with E.Coli. The lipid content increased gradually from 2 hrs to 24 hrs and decrease from 24 hrs to 48 hrs in male crabs challenged with E.coli and S.aureus.

Keywords: Barytelphusa guerini, Biochemical studies, E.coli, Staphylococcus aureus.