ECO-FRIENDLY SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM PUTATIVE MEDICINAL PLANT ANNONA RETICULATA

Veera Babu Nagati, Chandra Shakar Reddy Nallagouni, Rama Koyyati and Pratap Rudra Manthurpadigya*

ABSTRACT

Synthesis of silver nanoparticles using green method is an emerging aspect of nanoscience; it is safe and eco-friendly. Particularly medicinal plants used for synthesis gain prominent success in the metallic nanoparticle. In this work, silver nanoparticles were synthesized by using fresh leaves extract of Annona reticulata plant. The synthesized nanoparticles were characterized by UV-Visible Spectrophotometer, Fourier Transform Infrared (FTIR) spectroscopy; Scanning Electron Microscope (SEM) and Transmission Electron Microscopy (TEM). Results confirmed the formation silver nanoparticles and are spherical in shape and the average 50 nm in size. The biosynthesized silver nanoparticles were studied for their antibacterial activity against gram-positive bacteria Staphylococcus aureus, Micrococcus luteus, and gram-negative Escherichia coli, Pseudomonas putida bacteria. It was observed that synthesized silver nanoparticles have a maximum zone of inhibition for gram-positive bacteria Staphylococcus aureus followed by Micrococcus luteus bacteria.

Keywords: Biological synthesis, antibacterial studies, Transmission electron microscope, silver nanoparticles.