SOME RESISTANT GENES ASSOCIATED WITH DIARRHOEA IN RIVERS STATE

*Agi Vivian Nkemkanma, Samuel Douglas Abbey, Confidence Kinikanwo Wachukwu, Easter Godwin Nwokah and Ollor Ollor Amba

ABSTRACT

Extended-spectrum beta-lactamse (ESBL)-producing organisms are now increasing among clinical isolates worldwide and isolation and detection of ESBL- producing strains is crucial for the selection of most effective antibiotic for treatment of infections. Due to the increasing rise of antibiotic resistance to some commonly used antibiotics for diarrhoea treatment and the limited information about the epidemiology of ESBL producing bacteria in Rivers State, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia isolated from 120 diarrhoeic stool samples were investigated for the presence of resistance genes. In this study Plasmid DNA was extracted from bacteria and PCR amplification of the genes SHV, CTX-M and OXA was done using their specific primers. The result showed the presence of 100%, SHV, 10% CTX-M and 10% OXA genes amongst the enteric bacteria isolated from the diarrhoeic stool samples with SHV gene highest in prevalence. Therefore, proper management of prescription of antibiotics and also identification of ESBL producing bacteria in a community is important for effective prevention and treatment of infectious diseases.

Keywords: Extended-spectrum beta-lactamse, Antibiotic resistance, diarrhoea, bacteria.