PILOT AND ACUTE TOXICITY STUDIES ON TARTRAZINE IN ALBINO RATS
*Elekima Ibioku, Nwachuku Edna Ogechi and Nduka Nsirim
Pilot and acute toxicity studies were carried out to determine the LD100 and LD50 of tartrazine administered intraperitoneally and orally. A total of 68 albino rats weighing approximately 150g were used. 10 rats were used for the determination of LD100 of tartrazine administered intraperitoneally with doses of 0.0g/kg, 1.68g/kg, 3.33g/kg, 5.0g/kg, 6.67g/kg, 8.33g/kg, 10.0g/kg, 13.33g/kg, 15.0g/kg and 16.67g/kg. Likewise, 10 rats were also used in the determination of LD100 of tartrazine administered orally with doses of 0.0g/kg, 2.5g/kg, 5.0g/kg, 10.0g/kg, 15.0g/kg, 20.0g/kg, 22.5g/kg, 30.0g/kg, 35.0g/kg and 40.0g/kg. In the acute study, rats for intraperitoneal and oral administration were classified into six groups. Each group had a total of four rats. The intraperitoneally treated groups were designated Ai, Bi, Ci, Di, Ei and Fi and were treated with 0.0g/kg, 1.68g/kg, 3.33g/kg, 5.0g/kg, 6.67g/kg, 8.33g/kg respectively while that of oral were designated Ao, Bo, Co, Do, Eo and Fo were also treated with 0.0g/kg, 2.5g/kg, 5.0g/kg, 10.0g/kg, 15.0g/kg, 20.0g/kg respectively after LD100 determination. From studies, the LD100 were found to be 8.33g/kg and 20.0g/kg while the LD50 were found to 5.83g/kg and 11.25g/kg for intraperitoneally and orally treated rats respectively. Based on the LD50 obtained, tartrazine could be classified as practically non-toxic substance according Matsumura toxicity rating of chemical when administered intraperitoneally or orally.
Keywords: Pilot, Acute, toxicity, Tartrazine, LD100, LD50.
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