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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



*Elekima Ibioku, Nwachuku Edna Ogechi and Nduka Nsirim


Pilot and acute toxicity studies were carried out to determine the LD100 and LD50 of tartrazine administered intraperitoneally and orally. A total of 68 albino rats weighing approximately 150g were used. 10 rats were used for the determination of LD100 of tartrazine administered intraperitoneally with doses of 0.0g/kg, 1.68g/kg, 3.33g/kg, 5.0g/kg, 6.67g/kg, 8.33g/kg, 10.0g/kg, 13.33g/kg, 15.0g/kg and 16.67g/kg. Likewise, 10 rats were also used in the determination of LD100 of tartrazine administered orally with doses of 0.0g/kg, 2.5g/kg, 5.0g/kg, 10.0g/kg, 15.0g/kg, 20.0g/kg, 22.5g/kg, 30.0g/kg, 35.0g/kg and 40.0g/kg. In the acute study, rats for intraperitoneal and oral administration were classified into six groups. Each group had a total of four rats. The intraperitoneally treated groups were designated Ai, Bi, Ci, Di, Ei and Fi and were treated with 0.0g/kg, 1.68g/kg, 3.33g/kg, 5.0g/kg, 6.67g/kg, 8.33g/kg respectively while that of oral were designated Ao, Bo, Co, Do, Eo and Fo were also treated with 0.0g/kg, 2.5g/kg, 5.0g/kg, 10.0g/kg, 15.0g/kg, 20.0g/kg respectively after LD100 determination. From studies, the LD100 were found to be 8.33g/kg and 20.0g/kg while the LD50 were found to 5.83g/kg and 11.25g/kg for intraperitoneally and orally treated rats respectively. Based on the LD50 obtained, tartrazine could be classified as practically non-toxic substance according Matsumura toxicity rating of chemical when administered intraperitoneally or orally.

Keywords: Pilot, Acute, toxicity, Tartrazine, LD100, LD50.

[Full Text Article]



  • New Issue Published

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )