AN APPROACH ON BIOSYNTHESIS OF FIBRINOLYTIC ENZYME BY SUPPLEMENTATION OF TRACE METAL IONS FROM ASPERGILLUS JAPONICUM

Saroj Yadav and Siddalingeshwara K. G.*

ABSTRACT

Fibrinolytic enzymes are those enzymes that are able to digest fibrin, the major protein in blood clot formation. For therapeutic applications, three common fibrinolytic enzymes viz., streptokinase, urokinase and tissue plasminogen activators have been used with some degree of success. But they elicit undesired effects like internal haemorrhage, short half-life and unintended immune responses. The exploration of fibrinolytic enzyme is utmost important. Fibrinolytic enzyme production was investigated in the filamentous fungi Aspergillus japonicum KGSY 05 a soil isolate of different regions from Bangalore. The process economization were employed to achieve higher yield of Fibrinolytic enzyme through submerged fermentation (SmF) and here as have made an attempt to incorporate metal ions as a trace elements sources such as copper sulphate (CuSO4), iron sulphate (FeSO4), Zinc sulphate (ZnSO4) and magnesium sulphate (MgSO4) as a source to the production medium in the range of 0.01%, 0.02% and 0.03%. Iron sulphate and Zinc sulphate acted as best source of metal ions for the production of fibrinolytic enzyme at 0.02% at 72 hrs of fermentation period, the enzyme production observed was 112 IU and 105 IU respectively. Magnesium sulphate and Copper sulphate were less inducers of Fibrinolytic enzyme production and the enzyme production observed was 95 & 85 IU respectively at 0.01% for 72 hrs of fermentation period.

Keywords: Fibrinolytic Enzyme, Fibrin Plate, Submerged Fermentation and Metal Ion.