PROCESS ECONOMIZATION OF ORGANIC AND INORGANIC NITROGEN SOURCE IN SUBMERGED MEDIA FOR ALKALINE PROTEASE PRODUCTION FROM ASPERGILLUS AWAMORI

Radhika Pilli and Siddalingeshwara K. G.*

ABSTRACT

Protease constitutes a large and complex group of enzymes that plays an important nutritional and regulatory role in nature. Proteases are (physiologically) necessary for living organisms; they are ubiquitous and found in a wide diversity of sources. Protease production were carried out by using supplementation of organic and inorganic nitrogen sources such as Beef extract, Yeast extract and peptone at concentrations ranging from 0.25% to 1.25% with increments of 0.25% and also different inorganic nitrogen sources like Ammonium chloride and ammonium sulphate at concentrations ranging from 0.025% to 0.125% with increments of 0.025%. The peptone (1%) produced 3.15 IU were best organic nitrogen source and ammonium sulphate (0.025%) appear to be good inorganic nitrogen source under submerged fermentation process and showed 2.98 IU by using Aspergillus awamorii KGSR12.

Keywords: Protease, Organic Nitrogen, Inorganic Nitrogen, Submerged Fermentation.