ASSESSMENT OF VITAMINS, FLAVONOIDS AND PHENOLIC-COMPOUNDS BY HPLC IN THE FRUIT OF ELAEOCARPUS OBLONGUS

Raghunathan M.*

ABSTRACT

Background: Elaeocarpus oblongus wildly grown tree Western-Ghats, the ethnic peoples of the Nilgris district of Tamilnadu state, India are used the fruits for curative purpose. Fruits are edible; they were extremely cheapest resource for the control of rheumatism and body ache. Fruit containing seed is a kind of Rudraksha according Hindu mythology. Native peoples of Nilgiris district knew about the medicinal and nutritional values of the fruit. Objective: The current study indented to estimate the fat and water soluble vitamins, phenolics and flavanoids contents in the fruit pulp of Elaeocarpus oblongus (Elaeocarpaceae) by using HPLC methods. Qian H, Sheng M. (1998), suggested HPLC methods for analysing the nutritive values of natural products. Results: The results shown the vitamins A, D3 and E and K identified. Of these, A (Retinol Acetate) 1.817 mg/ gm, occurred in higher concentration followed by Vitamin K. 0.748 mg/ gm, Alpha -Tocopherol (E) 0.033 mg/ gm, Cholecalciferol, Vitamin (D3) 0.008 mg/ gm was found comparatively small level. B complex vitamins detected were niacin, pyridoxine, thiamine, and riboflavin. Amongst these, the highest quantity of water-soluble vitamins in the fruit pulp were Thiamine 0.334, followed by Riboflavin 0.012, Pyridoxine 0.007, and Niacin 0.002 mgs/gms respectively. Among the Phenolic acids, Gallic acid content was found more in amount 0.288 mg/ gm, followed by Ferulic-acid 0.009 mg/ gm, Caffeic acid 0.001 mg/ gm, Quercetin the plant flavanoid present in fruit pulp was 0.012 mg/gm, while all other flavonoids Rutin was 0.005 mg/ gm, are also in detectable amount. Conclusion: All these essential nutrients have played an important role in the day to day life of every human. The current study will be planned to be carried out GCMS analysis of fruit extracts, and Anti-nociceptive activity as well.

Keywords: Vikki, Bikki, Elaeocarpus oblongus, Vitamins, HPLC, Rudraksha, Flavanoids.