EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITION OF HYDROCOTYLE NEPALENSIS

Seok Hyeon Moon and Man Kyu Huh*

ABSTRACT

Objective: The objectives of this study were to determine antioxidant activities and lipoxygenase inhibition of ethanol extracts of Hydrocotyle nepalensis (Araliaceae) leaves and stems. Materials and Methods: The hydroxyl radical (-OH) can damage virtually all types of macromolecules: carbohydrates, nucleic acids (mutations), lipids (lipid peroxidation), and amino acids. The 1, 1- diphenyl 2-picrylhyorazyl (DPPH) is a well-known radical and a trap (scavenger) for other radicals. Results: The antioxidant activity of the H. nepalensis extracts was measured on the basis of the scavenging activity of the –OH and DPPH free radical. Hydroxyl radical scavenging activity of H. nepalensis was 78.8%. DPPH scavenging activity of leaves of H. nepalensis was evaluated at 8.0 mg/ml was 96.8%. Lipoxygenases (LOXs) constitute a heterogeneous family of lipid peroxidizing enzymes capable of oxygenating polyunsaturated fatty acids to their corresponding hydroperoxy derivatives. The inhibitory effect of 15-LOX by H. nepalensis was assayed using a Morgan microplate assay. The extract of H. nepalensis was 67.6% inhibitory effects on the activation of LOX. The IC50 values for OH activity, DPPH activity, and LOX inhibition were 14.61 μg/ml, 5.26 μg/ml, and 31.27 μg/ml, respectively. Conclusion: These results show that H. nepalensis has some phytochemical constituents which may be active against the free radicals (OH and DPPH) and lipoxygenase enzyme.

Keywords: Antioxidant activities, hydroxyl radical, 1, 1- diphenyl 2-picrylhyorazyl (DPPH), Hydrocotyle nepalensis, lipoxygenase.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )