( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



Mohamed A. Moustafa*, Doaa A. Ghareeb, Elsayed Elsayed Hafez, Bassiouny El-Gamal , Mohamed El-Sadanii


Lipopolysaccharide (LPS) induced the over- expression of a large amount of inflammatory mediators such as TNF-alpha and IFN-gamma which induced the transcription of inducible nitric oxide synthase (iNOS). L-Arginine modulates the action of NO and could be control the inflammation process. This study was conducted to elucidate the importance of L-Arginine as immunomodulator upon intraperitoneal injection of rats with potent inflammatory inducer, LPS and to deduce the exact mechanism by which L-arginine modulate or activate the immunity system either through induction of nitric oxide synthase (NOS) or arginase pathway. To evaluate these objectives, 5 groups, were intraperitoneally injected as follow; Control group), protection group: injected with 10 mg/kg L-Arginine for 7 days then once injected with 4mg/kg LPS, treatment group: injected with LPS followed by L-Arginine, Inhibition group: injected sequentially with LPS, L-Arginine and 10mg/kg NG nitro L-Arginyl methyl ester (L-NAME), and finally induction group injected with LPS only. Cytokines IL-6 and IL-1b levels were estimated by using ELISA while TNF alpha was estimated by qPCR. Furthermore, hepatic, renal functions and oxidative stress parameters were estimated colorimetric. Our results showed that, the level of total plasma nitrite was increased in both treatment and protection group and decreased in the inhibition group. Moreover, the level of pro-inflammatory cytokines (IL-1B, IL-6 and TNF alpha) were increased in the both induction and inhibition group and decreased in the treatment and protection groups. Altogether, L-Arginine plays an important role as an immunomodulator through NO-iNOS metabolic pathway.

Keywords: L-Arginine, Lipopolysaccharide, Pro-inflammatory cytokines, L-NAME, TNF-alpha, NO.

[Full Text Article]



  • New Issue Published

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    DECEMBER Issue has been successfully launched on 1 December 2021.

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )