EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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 ICV - 79.57

Abstract

FIRST REPORT ON SCREENING OF KERATINOLYTIC BACTERIA FROM SOIL SAMPLES OF POULTRY WASTE DUMP SITES OF WIDE DELHI-NCR REGION

Shruti Sinha*, Vandana Shrivastava and Abhishek Mathur

ABSTRACT

Keratin is an insoluble and fibrous structural protein that is a constituent of feathers and wool. The protein is abundantly available as a by-product. The degradative product represents a valuable source of proteins and amino acids that could be useful for animal feeds or as a source of nitrogen for plants. However, the keratin-containing substrates and materials have high mechanical stability and hence are difficult to be degraded by common proteases. The present study involves the isolation of feather-degrading bacterial cultures isolated from soil samples of poultry waste dump sites of New Delhi. About 10 samples of soil were collected from various places of Delhi (Old Delhi railway station; Rohini West; Chandni Chowk; Ghazipur Mandi), Ghaziabad (Old Ghaziabad; Pratap Vihar) and Noida (Sector -4; 15; 53; 137) of Delhi-NCR region. The isolation of bacterial cultures was performed on Nutrient agar. The pure cultures were separately isolated in sterilized conditions and were maintained as glycerol stock for further use. The results showed the isolation of 25 pure bacterial cultures from soil samples. These bacterial cultures were able to grown in media with keratin/feathers as the carbon and nitrogen source. Further these bacterial strains were screened for keratinase production on keratin-agar plates. Amongst, 25 bacterial cultures, 12 bacterial cultures were found positive for keratinase production. These bacterial cultures were interpreted as keratinophilic bacterial straisn. Thus these 12 bacterial cultures were considered for the study and the reference codes were allotted to such bacterial cultures, NCFT/KB/101 to NCFT/KB/112. These bacterial cultures were further morphologically and biochemically identified and were screened for gram staining. The present study is the first kind of study ever reported on the isolation and screening of bacterial cultures for keratinase production from soil samples of poultry waste dump sites of wide Delhi-NCR region.

Keywords: Bacterial strains, poultry waste dump sites, keratinase production, keratinolytic, Delhi-NCR region.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )