( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



*Neha Chhabra, Anita Solanki, Rajani Athawale and Supriya Mahajan


In this paper, an application of Design of Experiment (DoE) concept for the development of a simple yet precise reverse phase HPLC method for analysis of tetrahydrocurcumin in bulk drug and dermal formulations including nanoemulsion and liposomes is described. The method optimization was accomplished by applying multi-level full-factorial design model using Design Expert® (Version 10) software. 3 fators (F1, F2 and F3) and 2 responses (R1 and R2) were used viz mobile phase ratio of acetonitrile and methanol, flow rate, % v/v acetic acid, Retenton time and number of theoretical plates respectively to obtain the right combination that gave single sharp peak. The optimized HPLC conditions (acetonitrile and methanol in the ratio of 53:47, 0.26 % v/v with glacial acetic acid, at a flow rate of 0.6 ml/min and temperature maintained at 25˚C ± 0.5˚C gave single sharp peak at 4.5 minutes. The developed method was validated using ICH guidelines (for linearity, precision, robustness, Limit of quantification and limit of detection) and then analysed using ANOVA and 3-dimensional plots. Values of adequate precision 21.614 indicated that the model could be used to navigate the design space. “Prob>5” value less than 0.0500 indicated that the model terms were significant. It was concluded that the proposed method was simple, sensitive, and highly robust for routine analysis of bulk drug and other dermal formulations of tetrahydrocurcumin.

Keywords: Tetrahydrocurcumin, RP-HPLC, Method development, Validation, Full factorial design.

[Full Text Article]



  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 November 2021 Issue has been Published, Kindly check it on


    NOVEMBER Issue has been successfully launched on 1 November 2021.

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    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from  5.804 to 6.222, due to high quality Publication at International Level

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )