URINARY 8-OHDG (8-HYDROXY-2? -DEOXYGUANOSINE) AS BIOMARKER OF CANCER INDUCED OXIDATIVE DNA DAMAGE

Deependra Singh Raghuwanshi and *Reddy P. B.

ABSTRACT

Cancer induced oxidative DNA damage is subject of interest today. The oxidized bases like 8-hydroxy-2’-deoxyguanosine are formed due to DNA damage which is continuously excreted through urine. 8-OHdG (8-hydroxy-2′ -deoxyguanosine) is one of the major forms of free radical-induced oxidative cuts, and for that reason it has been extensively used as a biomarker for oxidative stress and carcinogenesis. The present study was intended to assess the oxidative DNA damage in lung cancer patients by using a urinary 8-OHdG as biomarker. A case study was carried out on 18 lung cancer patients (12 men and 6 women) who were undergoing chemotherapy. Patients were identified on the basis of various hospital records. Urinary 8-OHdG levels of lung cancer patients were estimated by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody. Eighteen urine samples of pulmonary cancer patients were collected in and around Nagda town (Ujjain, M.P.India) and were examined for 8-oxo-2′-deoxyguanosine by ELISA technique. The urinary 8-OHdG levels in lung cancer patients were significantly higher than in control (119.48 ± 13.14 ng/ml versus 5782.2 ± 195.3 ng/ml). We conclude that estimation of urinary 8-OHdG is easy and useful biomarker for the diagnosis of cancer induced DNA damage. In our opinion a follow-up of urinary 8-OHdG might be a useful future tool for the evaluation of DNA damage.

Keywords: Reactive oxygen species (ROS), 8-Hydroxydeoxyguanosine (8-OHdG), ELISA, Lung cancer.