A COMPARATIVE STUDY AND PHYTOCHEMICAL SCREENING, DPPH FREE RADICAL SCAVENGING AND IN-VITRO STUDY OF CYTOTOXIC ACTIVITY OF ANDROGRAPHIS PANNICULATE AND LEUCAS ASPERA ON MA104.

Ponnambalam Arun*, Kaveri Krishnasami, Palani Gunasekeran, Vidya Padmanabhan

ABSTRACT

Objective: To investigate the phytochemical, cytotoxicity, antioxidant activity of whole Leucas aspera and Andrographis panniculate alcoholic extract on MA104 cells in-vitro using MTT. Methods: Andrographis panniculate and Leucas aspera was extracted by absolute ethanol, chloroform, acetone and water. This extract was subjected to phytochemical screening (qualitative), followed by analysis for antioxidant activity (DPPH assay) and ethanol extract was used to investigate the cytotoxic activity of the A.panniculate and L.aspera by in-vitro using 3‐(4, 5‐dimethyl thiazol‐2yl)‐2,5‐diphenyl tetrazolium bromide (MTT). Results: Crude ethanolic extract of plant Leucas aspera and chloroform extract of Andrographis panniculate showed to be having antioxidant activity with IC50 value of 4.45μg/ml while compared with control ascorbic acid which had an IC50 value of 13.5μg/ml. The potency of the plant extract concentration has been calculated using percent decrease in number of viable cells in MA104 compared with control value. Conclusion: Based on the crude extract results obtained indicates the presence of phenolic compounds that serves as free radical may be a promising drug candidate that could be used in drug designing for diarrheal and various pharmacologic activities and presence of tumorigenic property indicates it can also be used as candidate to treat cancer after screening of the pure compound in the plant.

Keywords: Phytochemicals, Leucas aspera, Andrographis panniculate, DPPH, MA104, Cytotoxicity.