ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RIBAVIRIN IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
Sonali Paresh Mahaparale*, Rasika Pramod Karandikar, Kundan B. Bhalerao and Pallavi Vilas Kangone
A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for estimating stability of Ribavirin in tablet dosage form. Chemically, Ribavirin is 1-beta-D-Ribofuranosyl-1, 2, 4-triazole-3-carboxamide. The chromatographic separation of Ribavirin was achieved using C18 (250x4.6mm) column with a mobile phase containing a mixture of Acetonitrile: Water (60:40 v/v). The flow rate was 1ml/min and effluent was monitored at 218.0 nm. The retention time for Ribavirin was found to be 2.70 min. The relative standard deviation for intraday and interday precision in tablet was always less than 2%. The method was validated for linearity, range, precision, accuracy, specificity, selectivity, intermediate precision, ruggedness, robustness, stability and suitability.
Keywords: Ribavirin, Reverse phase HPLC, Accuracy, Precision, Robustness, LOD, LOQ, Specificity.
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