INHIBITORY EFFECT OF DPPH RADICAL SCAVENGING ACTIVITY AND HYDROXYL RADICALS (OH) ACTIVITY OF SOLANUM TUBEROSUM

Yong Lim MD, PhD and *Man Kyu Huh MD, PhD

ABSTRACT

This study aimed to investigate possible antioxidant activity of various concentration extracts of Solanum tuberosum. The explored items were 1- diphenyl 2-picrylhyorazyl (DPPH) activity and hydroxyl radicals (OH). DPPH scavenging activity of leaf extracts of S. tuberosum was evaluated 48.1% at 4.0 mg/ml and that of stem was 39.6% at same concentration. DPPH scavenging activity of tuber extracts of S. tuberosum was evaluated at 4.0 mg/ml was only 10.1% at same concentration. When the L-Ascorbic acid used as a control, extract for leaves of S. tuberosum was 63.7% effects on the activation of DPPH and those of stems and flowers were 52.4% and 28.5%, respectively. The highest OH activity was recorded in the leaf extract among three vegetative and one reproductive organs. OH activity of matured stems was 55.5% at 4.0 mg/ml and leaves, tubers, and flowers were 52.1%, 16.2, and 23.26% at same concentration, respectively. When the H2O2 used as a control, extract for stems of S. tuberosum was 71.6% effects on the activation of OH and those of leaves, tubers, and flowers were 67.3%, 20.9%, and 29.9%, respectively. These findings suggested that activity of DPPH enzymes and OH by extract from S. tuberosum might contribute to their antioxidant activities in biological and phytochemical examination.

Keywords: 1, 1- diphenyl 2-picrylhyorazyl (DPPH), hydroxyl radicals (OH), Solanum tuberosum.