EFFECT OF ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA ON OXIDATIVE STRESS INDUCED BY CEREBRAL ISCHEMIA-REPERFUSION IN RATS

Anshuman Trigunayat* and Shardendu Kumar Mishra

ABSTRACT

Restoration of blood flow to ischemic brain is linked with generation of reactive oxygen species. In Ayurveda, the medicinal properties of Tinospora cordifolia have been attributed to its anti-stress, antioxidant, neuroprotective, adaptogeneic and nootropic properties. The present study investigates the effect of ethanolic extract of T. cordifolia on acute cerebral ischemia-reperfusion in rats. Acute cerebral ischemia-reperfusion (30 min occlusion of bilateral common carotid arteries followed by 45 min reperfusion) in Charles Foster (C.F.) strain rats was produced following standard technique. Effect of Tinospora cordifolia on lipid peroxidation, superoxide dismutase (SOD) activity, ascorbic acid, cyclic AMP level and total tissue sulfhydryl (T-SH) group in for brain region in acute cerebral ischemia-reperfusion were evaluated. T. cordifolia pre-treatment (100 mg/kg p.o. for 7 days) attenuated the reperfusion induced biochemical alterations. The results suggest protective role of T. cordifolia in cerebral ischemia reperfusion injury.

Keywords: Ischemia, Reperfusion injury, Oxidative stress, Neuroprotection, Tinospor.