“STUDIES ON INHIBITION OF CALCIUM OXALATE CRYSTALLISATION BY USING PLANT EXTRACT AND SOIL MICROFLORA”

Nikhilesh Kulkarni*, Nikita Kapse, Rachana Pachori, Prithviraj Sadar

ABSTRACT

Kidneys are the vital organs have indispensable function in body physiology, it maintains proper balance of water and mineral as well as filtration and excretion of waste products it also control blood pressure and produce certain hormones. Due to chemical imbalance in body the kidneys are deposited by hard minerals which results in to (kidney stone) Urolithiasis. Approximately 85% of stones are composed predominantly of calcium oxalate. Keeping the vision to investigate the novel strategies for the prevention and treatment of kidney stone disease. The in-vitro calcium oxalate inhibitory potential, of plant extracts viz; Bryophyllum Pinnatum, Basil, Wheat Grass, and Lemon and of cell free extract of soil bacteria viz; Streptococcus, Enterococcus, Pseudomonas was analyzed separately; The inhibition of nucleation, aggregation and growth was investigated spectrophotometrically. The study revealed that both plant and cell free bacterial extract have the possible inhibitory effect on calcium oxalate for its crystal nucleation, aggregation and growth. Therefore study enlightens the beneficial use of plant & soil bacterial extracts for treatment of urolithiasis.

Keywords: Urolithiasis, Calcium Oxalate Crystallization, Nucleation, Aggregation, Growth and Medicinal Plants and Soil Bacterial Extracts.