ISOLATION AND PURIFICATION OF HYALURONIC ACID LIKE COMPONENTS FROM IPOMOEA BATATAS (SWEET POTATO)

Shlini P.*, Sana, Fathima Mahmood and Shambhavi Ullavi

ABSTRACT

Hyaluronic acid is a substance that is present in the human body. It is found in the most elevated fixations in liquids in the eyes and joints. Individuals take hyaluronic acid for different joint issue problems, including osteoarthritis. Hyaluronic acid is utilized in certain eye surgeries like, corneal transplantation, and repair of a disengaged retina and other eye wounds. Hyaluronic acid is additionally utilized as a lip filler in plastic surgery. A few people apply hyaluronic acid to the skin for mending wounds, cosmetics, skin ulcers, and as a lotion. Hyaluronic acid has been advanced as a "wellspring of youth." In the present investigation, Ipomoea batatas (sweet potato) was chosen for the study. The samples were extracted with methanol and chloroform. Goat brain and Visceral fluid as standard was chosen for the study. Estimation was carried out to check the presence of total carbohydrates, proteins and reducing sugars. Qualitative and quantitative studies were carried out which indicated the presence of hyaluronic acid like components in sweet potato and brain. Further ion exchange chromatography was carried using DEAE-Sepharose. The elution obtained from ion exchange showed a peak at 230nm which indicates the presence of hyaluronic acid like components. The peak eluents were further purified by gel permeation chromatography using Sephadex G-100. The result indicates the presence of hyaluronic acid like components. The results of the present work suggested that hyaluronic acid like components could be present in Sweet potato.

Keywords: Hyaluronic acid, Sweet potato, chromatography, Visceral fluid.