ANTIBIOTIC SUSCEPTIBILITY PATTERN OF PSEUDOMONAS SPECIES AT A TERTIARY CARE HOSPITAL IN CENTRAL INDIA.

Tore R. P*, Lichade S. B and Shrikhande S. N.

ABSTRACT

Introduction: Pseudomonas is widely distributed in nature. Intrinsic resistance to numerous antibiotics contribute to their virulence. Aims & Objectives: To isolate the Pseudomonas species from different clinical specimens and to study their antibiotic susceptibility pattern. Material & Methods: Study was conducted in Department of Microbiology, Indira Gandhi Govt. Medical College, Nagpur from August 2011 - August 2013. Pseudomonas isolates were processed to species level by standard procedures and antibiotic susceptibility was done according to CLSI guidelines 2011. Observations & Results: A total of 210 Pseudomonas strains were isolated and maximum 44% were from pus. Amongst them, 95% were P. aeruginos ,2.4% were P. putida, 1.4% were P.stutzeri and 0.95% were P. fluorescens. Amongst the 200 P . aeruginosa, least resistance was found to colistin (2.5%) and imipenem (21%) followed by piperacillin-tazobactum (25%) and amikacin (27%). Resistance to cephalosporines, aminoglycosides, tobramycin and netillin was in the range of 30 – 35%. Ciprofloxacin was 52% and norfloxacin was 67% resistant. Metallo-betalactamase (MBL) production was observed in 12% isolates. The Pseudomonas isolates other than P. aeruginosa, were more sensitive as compare to P. aeruginosa. They were 100% sensitive to colistin and imipenem followed by 80% sensitivity to piperacillin -tazobactum, 70% to amikacin & gentamycin, 60% to tobramycin & netillin and 50% to cephalosporines, quinolones and aztreonam.

Keywords: Pseudomonas species, antibiotic resistance, metallo-beta lactamase.