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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57



Prabhusaran Nagarajan*, Natarajaseenivasan Kalimuthusamy, Joseph Pushpa Innocent Danialas


Culturing Leptospira is the difficult and challenging task for the bacteriologists. But for maintaining leptospiral cultures and to cultivate this spirochete from the infective samples, it is mandatory to prepare the EMJH semisolid medium without any contaminations. There are more chances to get the EMJH medium contaminated. Some major reasons are summarized in this paper. More scientists working in leptospiral research confused themselves by observing the black deposits on the EMJH medium due to some metabolic process of the leptospires and few suggested contamination is the major reason for this issue. This controversy induced us to perform the laboratory test by taking three sets of EMJH semisolid medium. First set of medium inoculated with five leptospiral strains aseptically, second set inoculated with same five leptospiral strains as defined in first set in contaminatable manner and the third set without leptospiral inoculation but induced contamination by artificial open tube method. After incubation, the tubes were analyzed for Dinger’s ring formation and black pigments. The uncontaminated set of EMJH cultures showed Dinger’s ring only without any black pigmentation. Among the second set of medium, the tubes inoculated with Javanica, Patoc and Australis showed black pigments to black deposits and black threads found. This may be due to biochemical metabolic reactions by the contaminant mainly due to Bacillus sp (a common laboratory contaminant). More analysis required to incubate further, compare with more leptospiral strains and variations in EMJH semisolid medium composition to confirm the issue.

Keywords: Leptospira, EMJH semisolid medium, black pigments, contamination.

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia