DENGUE VIRUS-2 AND DENGUE VIRUS-3 CO-INFECTION IN THE SAME INDIVIDUAL-THE NOTORIOUS ALLIANCE OF TODAY
*Dr. Rajdeep Saha MD, Dr. Apoorbaa Roy, Dr. Dipika Menon Mukherjee, Dr. Rajyasri GuhaThakurta MD.
Dengue is one of the most important arboviral infections prevalent in India. Dengue virus belongs to the genus flavivirus and family flaviviridae. The principle vector is Aedes aegypti. The disease is caused by any of the five serotypes DENV 1, DENV 2, DENV 3, DENV 4 and DENV 5.This present study has been planned to observe the co-infection of an individual with serotypes DENV 2 and DENV 3 and assess the clinical complications arising due to this co-infection. A total of 4337 blood samples were collected from patients who were grouped into: fever less than 5 days and fever more than 5 days. Serum from these samples was tested using ELISA: NS1 (for fever <5days) and MAC (for fever >5days). The samples which tested positive were further tested using PCR for identification of serotypes. Total no. of samples taken was 4337. Out of which 1401 (32.3%) samples tested positive with ELISA (NS1/MAC). On performing PCR 1050 (74.9%) samples showed infection with a single serotype and 351(25%) samples showed bands in two or more regions indicating co-infection with multiple serotypes. Out of which 237 (67.5%) showed co-infection with DENV 2(Dengue virus type 2) and DENV 3(Dengue virus type 3) and the rest i.e. 114 (32.4%) tested positive for co-infection with other serotypes. Amongst these 237 cases: 228 developed DHF (Dengue Haemorrhagic Fever) and 09 developed DSS(Dengue Shock Syndrome). Hence we see in this outbreak of dengue there is increased incidence of complication when thereâ€™s a co-infection with DENV2 and DENV3.
Keywords: Dengue, Dengue virus type 2(DENV 2), Dengue virus type 3(DENV 3), ELISA.
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