SERUM HOMOCYSTEINE LEVELS IN CELIAC DISEASE PATIENTS

*Dr. Sindhu Sahito, Dr. Abed Elfattah Atieh, Dr. Love Kumar, Dr. Muhammad Fahad Pathan, Usama Abid and Ali Mohsin

ABSTRACT

Objective: To assess the serum homocysteine level in Celiac disease patients. Study Design and Setting: This was a observational study and was carried out at Department of Medicine and Gastroenterology Unit, Liaquat University Hospital Hyderabad from March 2016 to November 2016. Material and Methods: Total 90 cases were selected, out of them 45 cases had celiac disease and 45 were normal. All the diagnosed cases of Celiac disease either gender were incorporated. Careful clinical evaluation including a full medical history and clinical examination were done to confirm the diagnosis. All the participants were informed about advantages and disadvantages. Vein was engorged by a tourniquet applied above cubital fossa.10 ml of blood sample was collected from ante-cubital vein after application of sterilized alcohol swab. 5 ml was put in EDTA containing blood CP bottle and 5 ml in plain glass tube. Serum homocysteine levels were estimated by enzyme linked immunosorbent assay (ELISA) kit. Protein bound Hcy is first reduced to free Hcy, then it is catalyzed by the recombinant methionine, to produced H2S. The H2S reacts with chromophore and results are measured at 660nm. Total Hcy levels are measured proportional to the optical density. Results: Total 90 cases were compared according to serum homocysteine, out of them 45 were with celiac disease and 45 were normal without significant difference of age and gender. Serum homocysteine was significantly high 14.54±11.76 μM/L in cases having celiac disease as compare to controls 6.77±2.87 μM/Lvp-value of 0.0001. Serum homocysteine as categories of normal, mild elevation, moderate elevation and severe elevation. Mild and moderate elevations of serum Hcy in controls and cases were noted as 3 (6.6%) 0 (0%) vs. 11 (24.4%) and 8 (17.7%) respectively p-value =0.0001. Conclusion: We concluded that elevated serum homocysteine was significantly high in the in patients having Celiac disease and prevalence of elevated homocysteine was found in 42.2%.

Keywords: Frequency, serum homocysteine, celiac disease.