HEDERAGENIN AMELIORATES ANTIDIABETIC, ANTIHYPERLIPIDEMIC AND ANTIOXIDANT EFFECTS IN HIGH FAT DIET / STREPTOZOTOCIN-INDUCED DIABETES IN MICE

Vinitha Lakshmi.P*, Dhamotharan.Ra, Kalyan kumar.G, Srinivasa.H

ABSTRACT

The present study describes the antidiabetic effect of the hederagenin from Sapindus trifolitis inhibited dipeptidyl peptidase IV (DPP-IV) in vitro with an IC50 of 110.27±12.9 μg/ml. Hederagenin at doses of 200, 400 and 600 mg/kg p.o., also produced dose-dependent mean percent reductions of glucose excursion (AUC0–120 min) respectively in lean mice. However, even the highest dose of hederagenin did not alter normoglycemic condition. Hederagenin at dose of 200 mg/kg/day, p.o.for 28 days produced significant (p < 0.05) reduction in bodyweight, plasma glucose (PG), triglycerides (TG) total cholesterol (TC), BUN (Blood Urea Nitrogen) and CREA(creatinine) content in high-fat streptozotocin-induced diabetic mice. Hederagenin also improved oral glucose tolerance significantly (p < 0.05) reduced glucose excursion (AUC 0– 120 min) and significantly (p < 0.05) enhanced the endogenous antioxidant status when compared to diabetic control. Hederagenin preserved islet architecture and prevented hypertrophy of Bowman’s capsule as evident from the histopathology of pancreas and kidney. Hederagenin did not show any detectable haematological toxicity at therapeutic doses. In conclusion, Hederagenin exhibits antidiabetic effect possibly by inhibiting DPP-IV and improving antioxidant levels in high fat diet/ streptozotocin (HFD/STZ) diabetic mice.

Keywords: Sapindus trifolitis, hederagenin, antidiabetic, streptozotocin.