VALIDATION OF A UPLC METHOD WITH DIODE ARRAY DETECTION FOR THE DETERMINATION OF NOSCAPINE IN SYRUP DOSAGE FORM
Anas Rasheed* and Dr. Osman Ahmed
ABSTRACT
A specific, precise, accurate ultra pressure liquid chromatography (UPLC) method is developed for estimation of Noscapine in bulk drug and syrup dosage form. The method employed, with Hypersil BDS C18 (100 mm x 2.1 mm, 1.7 μm) in a gradient mode, with mobile phase of Octane sulphonic acid buffer : acetonitrile 35:65 %v/v. The flow rate was 1.0 ml/min and effluent was monitored at 260 nm. Retention time was found to be 2.050±0.880 min. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) etc. in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 20- 100 μg/ml respectively. The LOD and LOQ values for were found to be 2.098(μg/ml) and 6.3597(μg/ml) respectively. No chromatographic interference from syrup excipients and degradants were found. The proposed method was successfully used for estimation of Noscapine in syrup dosage form.
Keywords: Noscapine, syrup dosage form, UPLC method estimation.
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