TO STUDY THE EFFECT OF BASIC SOLVENT ON MERCAPTOPURINE BY USING UV-SPECTROPHOTOMETER
Rajashri Patil*, Mahananda Ghodke, Vishal Jadhav and Mukesh Mohite
Three precise and economical UV methods have been studied for estimation of Mercaptopurine in bulk formulation. Method A involves measurement of UV absorbance in Zero order derivative & Method B involves first order derivative are at 219 & 299 nm respectively. Method C deals with Area Under Curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 306-315 nm. The drug follows Beer-Lambert’s law in the concentration range of 1-10 μg/ml in all three methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed anhydrous solvent i.e. Sodium hydroxide and methods can be successfully applied for estimation of Mercaptopurine in routine analytical work.
Keywords: Mercaptopurine, Sodium hydroxide, Zero Order derivative, First order derivative, Area Under Curve method (AUC), UV Spectrophotometer.
[Full Text Article]